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Microalgal bioremediation of food-processing industrial wastewater under mixotrophic conditions: Kinetics

Suvidha Gupta,R. A. Pandey,Sanjay B. Pawar

《化学科学与工程前沿（英文）》 2016年 第10卷 第4期   页码 499-508 doi: 10.1007/s11705-016-1602-2

摘要： The microalgae were mixotrophically cultivated in an unsterilized and unfiltered raw food-processing industrial wastewater. Both inorganic carbon (CO -air) and organic carbon (wastewater) were provided simultaneously for microalgae growth. The aim of the study is to find out the utilization rates of total organic carbon (TOC) and chemical oxygen demand (COD) under mixotrophic conditions for a given waste water. About 90% reduction in TOC and COD were obtained for all dilutions of wastewater. Over 60% of nitrate and 40% of phosphate were consumed by microalgae from concentrated raw wastewater. This study shows that microalgae can use both organic and inorganic sources of carbon in more or less quantity under mixotrophic conditions. The growth of microalgae in food-processing industrial wastewater with all studied dilution factors, viz. zero (raw), 1.6 (dilution A), and 5 (dilution B) suggests that the freshwater requirement could be reduced substantially (20%–60%). The degradation kinetics also suggests that the microalgae cultivation on a high COD wastewater is feasible and scalable.

关键词： total organic carbon     wastewater bioremediation     kinetics     mixotrophic cultivation    

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Microalgae cultivation and culture medium recycling by a two-stage cultivation system

Xinfeng Wang, Lu Lin, Haifeng Lu, Zhidan Liu, Na Duan, Taili Dong, Hua Xiao, Baoming Li, Pei Xu

《环境科学与工程前沿（英文）》 2018年 第12卷 第6期 doi: 10.1007/s11783-018-1078-z

摘要：

A two-stage system was designed for microalgae cultivation and nutrients removal.

Two species of microalgae were cultivated for biomass production.

UF costed less than centrifuge for harvesting microalgae at small scale.

100% NH4+ of the wastewater was removed and met discharge requirement.

关键词： Microalgae harvest     Ultrafiltration     Poultry wastewater     Nutrient removal     Two stage cultivation    

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Effects of cultivation strategies on the cultivation of Chlorella

Xiaoya Liu, Yu Hong, Peirui Liu, Jingjing Zhan, Ran Yan

《环境科学与工程前沿（英文）》 2019年 第13卷 第5期 doi: 10.1007/s11783-019-1162-z

摘要： Heterotrophic cultivation caused high level of ROS and high lipids accumulation. HMTC is the best culture strategy for improving the microalgal biomass. Chlorella sp. HQ had great nutrient removal capacity under five culture strategies. The effects of cultivation strategies (including autotrophic cultivation (AC), heterotrophic cultivation (HC), fed-batch cultivation (FC), heterotrophic+ autotrophic two-stage cultivation (HATC), and heterotrophic+ mixotrophic two-stage cultivation (HMTC)) on the growth and lipid accumulation of Chlorella sp. HQ and its total nitrogen (TN) and total phosphorus (TP) removal in secondary effluent were investigated in column photoreactors. The results showed that the TN and TP removal rates ranged between 93.72%–95.82% and 92.73%–100%, respectively, under the five different strategies. The microalgal growth potential evaluated by the maximal growth rate (Rmax) was in the order of HMTC>HC>FC>AC>HATC. The values of biomass, total lipid yield, triacylglycerols (TAGs) yield, and total lipid content of the microalga cultivated in the last 5 d increased significantly, but the TAGs productivities of the five strategies were lower than those in the first 7 d. Compared with all the other cultivation strategies, the TAGs productivity and yield after 12 d of cultivation under the heterotrophic condition reached the highest values accompanying the highest level of intracellular reactive oxygen species (ROS), in which the TAGs yield reached 40.81 mg/L at the end of the cultivation period. The peaks in TAGs yield and ROS level suggested that HC was beneficial for lipids accumulation via regulating the cellular redox status and exerting ROS stress on microalgal cells. In summary, HMTC was the best cultivation strategy for improving the microalgal biomass and HC was the best strategy for microalgal TAGs accumulation to produce biodiesel.

关键词： Chlorella sp. HQ     Cultivation strategy     lipids     Nitrogen removal     Phosphorus removal     Reactive oxygen species    

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Cyanobacterial photo-driven mixotrophic metabolism and its advantages for biosynthesis

Ni Wan,Mary Abernathy,Joseph Kuo-Hsiang Tang,Yinjie J. Tang,Le You

《化学科学与工程前沿（英文）》 2015年 第9卷 第3期   页码 308-316 doi: 10.1007/s11705-015-1521-7

摘要： Cyanobacterium offers a promising chassis for phototrophic production of renewable chemicals. Although engineered cyanobacteria can achieve similar product carbon yields as heterotrophic microbial hosts, their production rate and titer under photoautotrophic conditions are 10 to 100 folds lower than those in fast growing Cyanobacterial factories face three indomitable bottlenecks. First, photosynthesis has limited ATP and NADPH generation rates. Second, CO fixation by ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) has poor efficiency. Third, CO mass transfer and light supply are deficient within large photobioreactors. On the other hand, cyanobacteria may employ organic substrates to promote phototrophic cell growth, N fixation, and metabolite synthesis. The photo-fermentations show enhanced photosynthesis, while CO loss from organic substrate degradation can be reused by the Calvin cycle. In addition, the plasticity of cyanobacterial pathways (e.g., oxidative pentose phosphate pathway and the TCA cycle) has been recently revealed to facilitate the catabolism. The use of cyanobacteria as “green ” could be a promising route to develop robust photo-biorefineries.

关键词： CO2 mass transfer     N2 fixation     photosystem     RuBisCO     the TCA cycle    

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Attached cultivation of

Victor M. Deantes-Espinosa, Tian-Yuan Zhang, Xiao-Xiong Wang, Yinhu Wu, Guo-Hua Dao, Hong-Ying Hu

《环境科学与工程前沿（英文）》 2019年 第13卷 第4期 doi: 10.1007/s11783-019-1141-4

摘要：

Attachment of Scenedesmus sp. LX1 was tested on certain materials.

A criterion for selection of materials was used to choose seven materials.

The amount of S. sp. LX1 attached on polyurethane foam was 51.74 mg/L.

Materials’ surface influenced the attachment of microalgae.

Hydrophilic and hydrophobic properties also affected the attachment of S. sp. LX1.

关键词： Attached cultivation     Microalgae     Material properties     Polyurethane     Scenedesmus    

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苹果栽培的世界性动向——提高果品质量

学士钊

《中国工程科学》 2001年 第3卷 第6期   页码 81-88

摘要：

由数量型向质量型转变，是苹果栽培的世界性动向。栽培者应该放弃只追求果品高额产量的过时做法，实施每公顷只产30 000 kg ，以达到最高的果品质量为目标，来保持果园的良性发展和增强果品抢占市场的优势。

关键词： 苹果栽培     世界性动向     果品质量    

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Dewatering of Scenedesmus Obliquus Cultivation Substrate with Microfiltration: Potential and Challenges

Marco Malaguti,Lorenzo Craveri,Francesco Ricceri,Vincenzo Riggio,Mariachiara Zanetti,Alberto Tiraferri,

《工程（英文）》 doi: 10.1016/j.eng.2023.07.010

摘要： In the microalgae harvesting process, which includes a step for dewatering the algal suspension, directly reusing extracted water in situ would decrease the freshwater footprint of cultivation systems. Among various algae harvesting techniques, membrane-based filtration has shown numerous advantages. This study evaluated the reuse of permeate streams derived from Scenedesmus obliquus (S. obliquus) biomass filtration under bench-scale and pilot-scale conditions. In particular, this study identified a series of challenges and mechanisms that influence the water reuse potential and the robustness of the membrane harvesting system. In a preliminary phase of this investigation, the health status of the initial biomass was found to have important implications for the harvesting performance and quality of the permeate stream to be reused; healthy biomass ensured better dewatering performance (i.e., higher water fluxes) and higher quality of the permeate water streams. A series of bench-scale filtration experiments with different combinations of cross-flow velocity and pressure values were performed to identify the operative conditions that would minimize energy requirements while maximizing water productivity. The optimal conditions, 2.2 m·s−1 and 1.4 bar (1 bar = 105 Pa), respectively, were then applied to drive pilot-scale microfiltration tests to reuse the collected permeate as a new cultivation medium for S. obliquus growth in a pilot-scale photo-bioreactor. The investigation revealed key differences between the behavior of the membrane systems at the two scales (bench and pilot). It indicated the potential for beneficial reuse of the permeate stream as the pilot-scale experiments ensured high harvesting performance and growth rates of biomass in permeate water that were highly similar to those recorded in the ideal cultivation medium. Finally, different nutrient reintegration protocols were investigated, revealing that both macro- and micronutrient levels are critical for the success of the reuse approach.

关键词： Scenedesmus obliquus     Microfiltration     Permeate reuse     Harvesting     Microalgae     Pilot-scale    

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我国法医学人才培养发展战略研究

侯一平 ,云利兵 ,诸虹 ,王茜 ,丛斌

《中国工程科学》 2019年 第21卷 第2期   页码 84-88 doi: 10.15302/J-SSCAE-2019.02.009

摘要：

本文通过研究国内外已有经验，分析我国法医学人才培养面临的挑战，提出促进法医学教育发展的政策建议：设立法医学一级学科，提升法医学科整体实力；建立法医学八年一贯制培养体系，创新法医学人才培养模式；增设法医学研究生专业学位，适应法治国家建设高素质应用型人才需求；建立确保鉴定人执业胜任能力和专业水平的继续教育制度；为临床医学生开设法医学课程，提升其法医学意识。

关键词： 法医学     医学教育     人才培养     发展战略    

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Drinking water biotic safety of particles and bacteria attached to fines in activated carbon process

CHEN Wei, LIN Tao, WANG Leilei

《环境科学与工程前沿（英文）》 2007年 第1卷 第3期   页码 280-285 doi: 10.1007/s11783-007-0047-8

摘要： In this paper, the drinking water biotic safety of particles and bacteria attached to fines in activated carbon process was investigated by actual treatment process and advanced treatment pilot trial with granular activated carbon. In the experiment, the particles were detected by IBR particle calculating instrument, the activated carbon fines were counted on the basis of the most probable number (MPN) with a microscope, the total number of bacteria was analyzed between the conventional agar culture medium and the one with R2A, and the bacteria attached to activated carbon fines was resolved by the homogenization technique. The experimental results showed that the average total number of particles was 205 CNT/mL in the activated carbon effluent during a filter cycle, of which the number of particles with sizes >2 μm was 77 CNT/mL more than the present particle control criterion of the American drinking water product standard (50 CNT/mL). The backwash of low density and long duration lowered particle number in the effluent. The MPN of activated carbon fines in the effluent was between 400 and 600 CNT/L, which accounted for less than 5 0 of the total particles from activated carbon filtration for a poor relative level ( = 0.34). The microorganisms in activated carbon effluent consisted mostly of heterotrophic bacillus and the total bacteria number was five times as high as that of the inflow, i.e. the effluent from sand filter. The actual bacteria number may be truly indicated by the detection technique with R2A culture medium compared with the traditional agar cultivation. The inactivation efficiency of bacteria attached to activated carbon fines was less than 40% under 1.1 mg/L of chlorine contacting for 40 min. Results showed that the particles and bacteria attached to activated carbon fines may influence drinking water biotic safety, and that the effective control measures need to be further investigated.

关键词： particle number     process     cultivation     activated     relative    

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哈密瓜南移东进生态育种与有机生态型无土栽培技术研究

吴明珠,伊鸿平,冯炯鑫,艾尔肯,张永兵

《中国工程科学》 2000年 第2卷 第8期   页码 83-88

摘要：

脆肉型哈密瓜原产新疆大漠干旱或半干旱地区，种植范围狭窄。经过8年16季南、北生态育种的探索研究，利用多种生态地区的亲本杂交、回交，核辐射诱变以及基因工程等手段结合常规选育，已育出8个哈密瓜改良品种（系）和3个单性花自交系，可在我国潮湿多雨、弱光照的东南部采用大棚有机生态型无土栽培方式种植，为生产无公害绿色食品、"安全甜瓜"开创了美景。

关键词： 哈密瓜     生态育种     核辐射诱变     基因工程     有机生态型     无土栽培     单性花    

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南方双季稻区冬油菜早熟品种选育和机械栽培研究进展

官春云,陈社员,吴明亮

《中国工程科学》 2010年 第12卷 第2期   页码 4-10

摘要：

为促进南方双季稻区冬油菜生产的发展，选育出油菜早熟品种和实行机械栽培是生产急需解决的两大问题。课题组经过近5年的系统研究，现已成功选育出5个早熟、双低、抗病、高含油量的油菜新组合（品系）。成功研制了适合油菜免耕（浅耕）直播要求的2BYF（D）—6型油菜免耕直播联合播种机，并配套研究了适合直播油菜要求的一次性施用控释肥；同时配合农艺上的催熟剂的开发与使用，研制了与高密度早熟油菜品种相配套的4YC-200油菜联合收割机。形成了在南方双季稻区机械化种植油菜农艺操作规程。通过以上集成技术的实施，实现了油菜种植成本降低，种植面积扩大，油菜产量和种植经济效益的提高，试验测定，通过机械化种植油菜，每公顷纯收入可增至4 500元以上。

关键词： 双季稻     油菜     早熟品种     机械栽培     控释肥     催熟剂    

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Cultivation of sp. HQ in inland saline-alkaline water under different light qualities

《环境科学与工程前沿（英文）》 2022年 第16卷 第4期 doi: 10.1007/s11783-021-1479-2

摘要：

• Optimal growth of Chlorella in inland saline-alkaline water was achieved by blue LED.

关键词： Light quality     Chlorella     Inland saline-alkaline water     Fatty acid     Biodiesel property    

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一种兼有增产和环保作用的耕作制度

陈国平

《中国工程科学》 2000年 第2卷 第1期   页码 87-89

摘要：

在干旱、半干旱地区实行免耕覆盖播种耕作制度，可减少蒸发，最大限度地保蓄上一年夏秋降雨，保证全苗，增加产量。同时，由于地面坚实，在秸杆保护，可减少沙尘暴天气；而在夏季，可增加水分的渗入，减缓地面径流，防止土壤冲刷造成河流泥沙淤积，是一项一举三得的重要措施。

关键词： 秸杆覆盖     沙尘暴     土壤冲刷     增加产量    

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航天工程科技国际化人才培养体系构建与实践

陈国宇,卢俊,黄昕,赵晟,张伟

《中国工程科学》 2019年 第21卷 第4期   页码 118-123 doi: 10.15302/J-SSCAE-2019.04.018

摘要：

在构建“人类命运共同体”和“一带一路”伟大倡议的历史背景下，国际航天合作有助于增进与沿线国家的共识，有助于实现互利共赢、共同发展。随着国际航天合作的不断发展，中国航天需要一支能够满足航天事业跨越式发展、具有较大影响力的航天英才队伍。本文给出航天工程科技国际化人才培养内涵，以中国航天科技集团有限公司第五研究院航天工程科技国际化人才培养为例，重点阐述航天工程科技国际化人才培养体系构建及实施,给出航天工程科技国际化人才培养体系实施效果。
 

关键词： 航天工程     系统工程     国际化     人才培养    

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大规模培养光合生物的光生物反应器设计 Review

黄青山, 蒋夫花, 王连洲, 杨超

《工程（英文）》 2017年 第3卷 第3期   页码 318-329 doi: 10.1016/J.ENG.2017.03.020

摘要：

光合微生物是生产环境友好的目标产品的重要生物资源，光生物反应器是实现这些过程的重要工具。目前，用于光催化的光生物反应器的设计具有挑战性，大多数光反应器仍然采用半经验的方法进行设计和放大。即使对于高效的光生物反应器，由于缺乏对光的传播、流体动力学、传质、细胞生长之间耦合作用的深刻了解，依靠半经验方法设计的反应器具有投资和运行成本高、使用寿命短的缺点，因此迄今为止尚没有一款合适的光生物反应器可用于光合微生物的大规模培养。首先，本文概述了影响光生物反应器性能的关键参数包括光、混合、传质、温度、pH 值、投资和运行成本等，强调了在商业化培养中光生物反应器的寿命、清洁成本和温度控制的重要性。然后，推荐了四种( 即管式光生物反应器、塑料袋式光生物反应器、柱状气升环流式光生物反应器和平板气升环流式光反应器) 可进行光合微生物大规模培养的光生物反应器。最后，阐述了采用计算流体力学这一有力工具进行光生物反应器建模从而实现光生物反应器的理性设计，并分析了当今数学建模的难点，展示了基于机理模型设计光生物反应器的发展前景。

关键词： 光生物反应器     太阳能     光合成     流体动力学     闪光效应    

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标题 作者 时间 类型 操作

Microalgal bioremediation of food-processing industrial wastewater under mixotrophic conditions: Kinetics

Suvidha Gupta,R. A. Pandey,Sanjay B. Pawar

期刊论文

Microalgae cultivation and culture medium recycling by a two-stage cultivation system

Xinfeng Wang, Lu Lin, Haifeng Lu, Zhidan Liu, Na Duan, Taili Dong, Hua Xiao, Baoming Li, Pei Xu

期刊论文

Effects of cultivation strategies on the cultivation of Chlorella

Xiaoya Liu, Yu Hong, Peirui Liu, Jingjing Zhan, Ran Yan

期刊论文

Cyanobacterial photo-driven mixotrophic metabolism and its advantages for biosynthesis

Ni Wan,Mary Abernathy,Joseph Kuo-Hsiang Tang,Yinjie J. Tang,Le You

期刊论文

Attached cultivation of

Victor M. Deantes-Espinosa, Tian-Yuan Zhang, Xiao-Xiong Wang, Yinhu Wu, Guo-Hua Dao, Hong-Ying Hu

期刊论文

苹果栽培的世界性动向——提高果品质量

学士钊

期刊论文

Dewatering of Scenedesmus Obliquus Cultivation Substrate with Microfiltration: Potential and Challenges

Marco Malaguti,Lorenzo Craveri,Francesco Ricceri,Vincenzo Riggio,Mariachiara Zanetti,Alberto Tiraferri,

期刊论文

我国法医学人才培养发展战略研究

侯一平 ,云利兵 ,诸虹 ,王茜 ,丛斌

期刊论文

Drinking water biotic safety of particles and bacteria attached to fines in activated carbon process

CHEN Wei, LIN Tao, WANG Leilei

期刊论文

哈密瓜南移东进生态育种与有机生态型无土栽培技术研究

吴明珠,伊鸿平,冯炯鑫,艾尔肯,张永兵

期刊论文

南方双季稻区冬油菜早熟品种选育和机械栽培研究进展

官春云,陈社员,吴明亮

期刊论文

Cultivation of sp. HQ in inland saline-alkaline water under different light qualities

期刊论文

一种兼有增产和环保作用的耕作制度

陈国平

期刊论文

航天工程科技国际化人才培养体系构建与实践

陈国宇,卢俊,黄昕,赵晟,张伟

期刊论文

大规模培养光合生物的光生物反应器设计

黄青山, 蒋夫花, 王连洲, 杨超

期刊论文